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    ATCC 3461 raw264 7 cells atcc cat
    3461 Raw264 7 Cells Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3461+raw264+7+cells+atcc+cat/RAW+264%2E7/pm40669456-148-112-115
    Average 99 stars, based on 24299 article reviews
    3461 raw264 7 cells atcc cat - by Bioz Stars, 2026-10
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    Luciferase:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Reporter Gene Assay:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Western Blot:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Polymerase Chain Reaction:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Recombinant:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Plasmid Preparation:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Software:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Chromatography:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Mass Spectrometry:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..

    Real-time Polymerase Chain Reaction:

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 Article ll OPEN ACCESS ..

    Article Title: A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#B2064 NaOH Sigma-Aldrich Cat#S5881 Citrate Sigma-Aldrich Cat#V900095 EDTA Sigma-Aldrich Cat#324503 10-kDa ultrafilter column Sigma-Aldrich Cat#PLGC02510 0.45 μm filter Sigma-Aldrich Cat#HVLP02500 Skimmed milk Sigma-Aldrich Cat#1.15338.0500 β-mercaptoethanol Santa Cruz Cat#sc-202966 Puromycin Santa Cruz Cat#sc-108071 Polybrene Santa Cruz Cat#sc-134220 c-di-GMP InvivoGen Cat#tlrl-nacdg 3′3′-cGAMP Abcam Cat#ab144865 PMA Abcam Cat#ab120297 c-di-AMP Acmec Cat#X80635 2′3′-cGAMP APExBIO Cat#B8362 MSA-2 Med Chem Express Cat#HY-136927 SR-717 Med Chem Express Cat#HY-131454 ABZI Med Chem Express Cat#HY-123943 DMXAA Med Chem Express Cat#HY-10964 Honokiol Med Chem Express Cat#HY-N0003 MCE FDA-approved Drug Library-1724 cmpds Med Chem Express Cat#HY-L021 Anti-virus Compound Library-508 cmpds Med Chem Express Cat#HY-L001 MetaSci Human Metabolite Library-916 cmpds MetaSci Cat#HML 12.99 Collagenase/Dispase Roche Cat#11097113001 DNase I Roche Cat#10104159001 Corn oil Beyotime Cat#ST2308 SDS Beyotime Cat#ST2681 Kanamycin Beyotime Cat#ST101 Triton X-100 Beyotime Cat#P0096 Coomassie blue Beyotime Cat#ST1119 Paraformaldehyde Beyotime Cat#P0099 7-AAD viability staining solution Selleck Cat#420404 ProLong Gold antifade reagent Invitrogen Cat#P36982 Trizol Invitrogen Cat#15596026 Lipofectamine 3000 Invitrogen Cat#L3000015 Ni-NTA Smart-Lifesciences Cat#SA004100 DMEM Gibco Cat#C11965500BT RPMI-1640 Gibco Cat#C11875500BT Fetal Bovine Serum Gibco Cat#A5669701 Penicillin Streptomycin Gibco Cat#15140-122 0.25% Trypsin-EDTA Gibco Cat#25200-072 HBSS Gibco Cat#14175095 PBS Gibco Cat#C10010500BT Matrigel BD Biosciences Cat#356234 Mouse CD16/CD32 BD Biosciences Cat#553141 Saponin-containing buffer BD Biosciences Cat#554722 Optimal cutting temperature (O.C.T.) compound Tissue-Tek Cat#4583 Poly (dA:dT) InvivoGen Cat#tlrl-patn (Continued on next page) e2 Cell Reports Methods 5, 101106, July 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays HiLoad 16/600 Superdex 200 pg column GE healthcare Cat#GE28-9893-35 Superdex 200 Increase 10/300 GL column GE healthcare Cat#GE28-9909-44 Dual Luciferase Reporter Gene Assay Kit Yeasen Cat#11402ES60 ELISA kits for detecting IFN-β Jiangsu Meibiao Cat#MB-6317A ELISA kits for detecting TNF-α Jiangsu Meibiao Cat#MB-2868A ELISA kits for detecting IL-6 Jiangsu Meibiao Cat#MB-2899A Deposited data Crystal structure of STING LBD in complex with honokiol RCSB Protein DataBank https://www.rcsb.org/structure/8Z37 Uncropped immunoblotting data and Excel datasheets for graphs Mendeley Data https://data.mendeley.com/ datasets/skv4hchsss/1 Experimental models: Cell lines B16F10 cells Cell Bank/Stem Cell Bank, SIBCB Cat#TCM36 MC38 cells Sigma-Aldrich Cat#SCC172 THP-1 cells Cell Bank/Stem Cell Bank, SIBCB Cat#SCSP-567 E0771 cells ATCC CatCRL-3461 RAW264.7 cells ATCC Cat#TIB-71 293T cells ATCC Cat#CRL-3216 Experimental models: Organisms/strains Sting+/− C57BL/6J mice GemPharmatech Cat#T012747 STING-humanized C57BL/6J mice GemPharmatech Cat#T049781 Wild-type C57BL/6J mice GemPharmatech Cat#N000295 Oligonucleotides PCR primers and DNA oligonucleotides This Paper Table S3 Recombinant DNA pET28a vector Sigma-Aldrich Cat#69864 pET28a-FiSL This paper N/A pET28a-dFiSL This paper N/A pET28a-hSTING-LBD This paper N/A pET28a-mSTING-LBD This paper N/A LentiCRISPRv2 vector Addgene Cat#98290 LentiCRISPRv2-non-target gRNA This paper N/A LentiCRISPRv2-STING gRNA This paper N/A LentiCRISPRv2-Sting gRNA This paper N/A pMD2.G plasmid Addgene Cat#12259 psPAX2 plasmid Addgene Cat#12260 Software and algorithms GraphPad Prism (Version 8.0.2) GraphPad Software http://www.graphpad.com Rstudio (Version 4.1.3) Posit Software https://posit.co/download/ rstudio-desktop/ PHENIX (Version 1.18.2–3874) Phenix Industrial Consortium https://phenix-online.org/ Pymol (Version 1.3) Schrödinger http://www.pymol.org Imaris (Version 9.0.1) Oxford Instruments http://www.imaris.com FlowJo (Version 10.8.1) Becton, Dickinson & Company https://www.flowjo.com/ Other Liquid chromatography/Quadrupole-TOF mass spectrometer Agilent 6530 https://www.agilent.com.cn/ ABI Q7 Fast Real Time PCR System Applied Biosystems https://www.thermofisher.cn/ BD LSRFortessa BD Biosciences https://www.bdbiosciences.com Cell Reports Methods 5, 101106, July 21, 2025 e3 ..



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